Actions

Download Analytics Citations

Export to: EndNote  |  Zotero  |  Mendeley

Collections

This file is not currently in any collections.

Bioengineering breast cancer invasion in vitro through enhancing the value of existing breast cancer epithelial lineages [dataset] Open Access

Breast cancer incidence is rising globally. Emerging literature implicates cancer cell interactions with their surrounding microenvironment as a clinically significant driver of cell migration and metas-tasis. Current in vitro modelling approaches often rely on basic two-dimensional culture practises, a microenvironment far removed from the native tissue. This simplicity fails to recapitulate key as-pects of the tumour environment, that are critical to disease progression. This study has built upon three-dimensional culture technology, to describe a breast cancer invasion assay with enhanced complexity compared with standard approaches. Three key breast epithelial cell lines including: MCF-10A, MCF-7 and MDA-MB-231 representative of healthy tissue, early stage, and metastatic cancer respectively, were all applied to in vitro systems of varying complexity. Through enhancing the geometry of the culture system, and inclusion of stromal populations, we have successfully im-proved breast cancer invasion modelling in vitro exemplified by a demonstrated response to a test compound. The incorporation of a tissue-specific stromal foundation upon which each epithelial cell line was cultured, allowed for interaction between neosynthesised extracellular matrix stromal and epithelial cell types, and a dense, physical network for cancer cells to migrate through. This has in-troduced several microenvironmental factors consistent with the native tissue but lacking in other more simplistic culture systems and has enhanced the value of commonplace cancer cell lines. This novel bioengineered culture system also lends itself to in-depth fundamental mechanistic insights into aspects of cancer cell biology, including interactions with the tissue environment and role of cancer associated fibroblasts in disease progression.

Descriptions

Resource type

Dataset

Contributors

Data collector: Webb, Ethan ¹
Creator: Goncalves, Kirsty ¹
Contact person: Goncalves, Kirsty ¹
Data curator: Goncalves, Kirsty ¹
Editor: Goncalves, Kirsty ¹
Contact person: Przyborski, Stefan ²
Editor: Przyborski, Stefan ²

¹ Durham University, UK
² Durham University, UK, Reprocell Europe Ltd. Glasgow

Funder

Research methods

Other description

Dataset consists of images in TIFF format.

Keyword

cancer associated fibroblasts
tissue bioengineering
extracellular matrix
breast cancer
migration
invasion
in vitro

Subject

Breast--Cancer--Research
Bioengineering--Research

Location

Language

Cited in

Identifier

ark:/32150/r1gt54kn09q
doi:10.15128/r1gt54kn09q

Rights

Creative Commons Attribution 4.0 International (CC BY)

Publisher

Durham University

Date Created

File Details

Depositor

K.E. Goncalves

Date Uploaded

2 October 2026, 09:10:34

Date Modified

2 October 2026, 11:10:06

Audit Status

Audits have not yet been run on this file.

Characterization

File format: zip (ZIP Format)

Mime type: application/zip

File size: 185667346

Last modified: 2026:10:02 10:01:46+01:00

Filename: Breast Cancer Paper Data.zip

Original checksum: 4e8a47ed37296b32a3cfbbbe1a113e60