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Freeform based Confocal Pushbroom Hyperspectral Imager for Biological Imaging Applications [dataset] Open Access

In this paper, we present a novel push-broom hyperspectral imaging instrument based upon a purpose built reflective optical architecture. Featuring a custom-built freeform blazed diffraction grating, two diffraction orders are used to maximise throughput over an extended spectral range of 500-1700 nm. The reflective scanning optics provide a high optical throughput throughout the spectral bandwidth, whilst enabling fast scan speeds for investigating evolving processes. In addition, a confocal illumination system is integrated so that only the instantaneously imaged line is illuminated at each point of an acquisition. This significantly reduces light exposure to the sample, providing benefits especially for photosensitive applications. We demonstrate the performance of the instrument by a time lapsed observation of a drying leaf, noting functional changes including pigment breakdown, structure and water content.

Descriptions

Resource type

Dataset

Contributors

Contact person: Graham, Christopher ¹
Creator: Zhang, Lai ¹
Bourgenot, Cyril ¹
Girkin, John ¹

¹ Durham University, UK

Funder

Engineering and Physical Sciences Research Council
Medical Research Council

Research methods

Other description

Keyword

hyperspectral imaging
confocal illumination
biological imaging
freeform optics

Subject

Hyperspectral imaging
Confocal microscopy
Three-dimensional imaging in biology

Location

Language

Cited in

Identifier

ark:/32150/r2zg64tk978
doi:10.15128/r2zg64tk978

Rights

Creative Commons Attribution 4.0 International (CC BY)

Publisher

Durham University

Date Created

File Details

Depositor

L. Zhang

Date Uploaded

27 August 2026, 16:08:36

Date Modified

8 September 2026, 13:09:44

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File format: zip (ZIP Format)

Mime type: application/zip

File size: 8618151639

Last modified: 2026:08:27 17:16:30+01:00

Filename: supporting data_HSI.zip

Original checksum: 06bcb6048d1cab5548227b47f4f360ae